abstract：:This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinov...

journal_title：Journal of virology

authors： Gerin JL,Richter WR,Fenters JD,Holper JC

更新日期：1968-09-01 00:00:00

abstract：:Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell...

journal_title：Journal of virology

authors： Miller G,Enders JF

更新日期：1968-08-01 00:00:00

abstract：:Strains of Bacillus that harbor defective phage PBSX were found to be insensitive to SP-10(C), although the phage adsorbed to these insensitive strains. Strains that did not carry the phage were sensitive to SP-10(C). B. subtilis 168 ind(-), which can be tranduced by SP-10(C) but is nonpermissive for the phage, was re...

journal_title：Journal of virology

authors： Goldberg ID,Bryan T

更新日期：1968-08-01 00:00:00

abstract：:Phage P22 can integrate as prophage into a recombination-deficient (Rec(-)) strain of Salmonella typhimurium. At 37 C, the integration efficiency is only 10% that in Rec(+) infection, but at 25 C the efficiencies in Rec(-) and Rec(+) hosts are similar. Rec(-) lysogens cannot be induced by ultraviolet irradiation or by...

journal_title：Journal of virology

authors： Wing JP

更新日期：1968-07-01 00:00:00

abstract：:A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2...

journal_title：Journal of virology

authors： Thorne CB

更新日期：1968-07-01 00:00:00

abstract：:The ribonucleic acid (RNA) from Western equine encephalomyelitis (WEE) virions sedimented through sucrose gradients with a sedimentation coefficient of 40S. Another viral RNA which was always associated with infected cells possessed a sedimentation coefficient of 26S. Both 40S and 26S RNA had identical base compositio...

journal_title：Journal of virology

authors： Sreevalsan T,Lockart RZ Jr,Dodson ML Jr,Hartman KA

更新日期：1968-06-01 00:00:00

abstract：:The treatment of bacteriophage T4D with 2-hydroxy-5-nitrobenzyl bromide, a specific reagent for alkylating the indole ring of tryptophan residues, converts these particles from a cofactor-independent form to a cofactor-sensitive form. These treated T4D particles phenotypically resemble T4B particles in certain respect...

journal_title：Journal of virology

authors： Gamow RI,Kozloff LM

更新日期：1968-05-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing pro...

journal_title：Journal of virology

authors： Jungwirth C,Launer J

更新日期：1968-05-01 00:00:00

abstract：:Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, o...

journal_title：Journal of virology

authors： De Tkaczevski L,De Harven E,Friend C

更新日期：1968-04-01 00:00:00

abstract：:Experiments with St. Louis encephalitis (SLE) virus have shown that neutral red enhances the plaque size in duck embryo cell cultures. This may represent a new method for genetic studies of SLE virus population. In a mosquito pool specimen NR(+) and NR(-) particles were demonstrated. By intracerebral passage in mice, ...

journal_title：Journal of virology

authors： Marcovici M,Prier JE

更新日期：1968-03-01 00:00:00

abstract：:After incubation at 37 C for 2 or more days, Basal Medium Eagle (BME) supplemented with 25% fetal calf serum (FCS) was not able to support adequate growth of EB3 and other lines of Burkitt tumor cells. The medium did, however, increase, by a factor of about 10, the number of cells synthesizing herpes-type virus with w...

journal_title：Journal of virology

authors： Henle W,Henle G

更新日期：1968-03-01 00:00:00

abstract：:The interferon response elicited by Salmonella typhimurium mutants in mice is not dependent on the presence of a complete cell wall lipopolysaccharide. In fact, a mutant (G30/C21) which has lost all the polysaccharide side chains and sugars of the O antigen and contains only 2-keto-3-deoxyoctonate and lipid is indisti...

journal_title：Journal of virology

authors： Youngner JS,Feingold DS

更新日期：1967-12-01 00:00:00

abstract：:New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorptio...

journal_title：Journal of virology

authors： Reimer CB,Baker RS,Van Frank RM,Newlin TE,Cline GB,Anderson NG

更新日期：1967-12-01 00:00:00

abstract：:A technique of continuous filtration of T4-infected Escherichia coli has been devised to study the phenomenon of lysis inhibition. Studies using this technique revealed that the length of the lysis delay caused by superinfection can attain only certain discrete values, which for low average multiplicity of superinfect...

journal_title：Journal of virology

authors： Bode W

更新日期：1967-10-01 00:00:00

abstract：:Simian virus 40 (SV40) infection of human diploid cells failed to cause an enhanced production of thymidine kinase during the first 10 days after infection. Thymidine kinase activities from extracts of SV40-transformed cultures (human or simian) were considerably higher than the activity levels in extracts from the no...

journal_title：Journal of virology

authors： Carp RI

更新日期：1967-10-01 00:00:00

abstract：:The incorporation of (3)H-thymidine ((3)H-dT) into deoxyribonucleic acid (DNA) has been studied in uninfected confluent monolayer cultures of monkey kidney and mouse kidney cells, simian virus 40 (SV40)-infected cells, and in SV40-transformed mouse kidney cells. Radioautographic measurements revealed that during the p...

journal_title：Journal of virology

authors： Kit S,De Torres RA,Dubbs DR,Salvi ML

更新日期：1967-08-01 00:00:00

abstract：:The presence of complement-fixing (CF) antibody reactive with T antigen and with viral C antigen in hamsters bearing adenovirus 12-induced tumors has been confirmed. Antibody activity in serum obtained at a time when the host was bearing large tumors was found to be associated exclusively with 7S immunoglobulins. Two ...

journal_title：Journal of virology

authors： Fugmann RA,Sigel MM

更新日期：1967-08-01 00:00:00

abstract：:From a stock of varkappa phage grown on Salmonella, a host-range mutant which attacks Escherichia coli was isolated. As in the case of Salmonella, only motile strains of E. coli are sensitive to varkappa. The phage shows an eclipse period of 35 min and a minimal latent period of 52 min. The adsorption rate constant is...

journal_title：Journal of virology

authors： Schade S,Adler J

更新日期：1967-06-01 00:00:00

abstract：:Purified replicative form (RF) and replicative intermediate (RI) prepared from Escherichia coli cells infected with the ribonucleic acid (RNA) bacteriophage R17 were denatured with dimethyl sulfoxide at 37 C or in aqueous solvents of low ionic strength at 97 C. Denaturation was demonstrated for RF and RI by an increas...

journal_title：Journal of virology

authors： Franklin RM

更新日期：1967-06-01 00:00:00

abstract：:Encephalomyocarditis (EMC) virus (K-2 strain) was separated into two distinct peaks of optical density by chromatography on calcium phosphate, eluting at 0.17 and 0.31 m, respectively. The virus in these peaks could not be distinguished by infectivity (plaque-forming units), hemagglutinin activity, electron microscopi...

journal_title：Journal of virology

authors： Burness AT

更新日期：1967-04-01 00:00:00

abstract：:Adenovirus type 12 (Huie) inoculated into cultures of primary whole rat embryo produced foci of morphologically altered cells. The number and identification of these transformed areas was dependent upon the calcium concentration of the medium; more foci appeared in 0.1 mm than in 1.8 mm calcium. Cell lines derived fro...

journal_title：Journal of virology

authors： Freeman AE,Black PH,Wolford R,Huebner RJ

更新日期：1967-04-01 00:00:00

abstract：:The morphology of Safferman's virus of blue-green algae (phycovirus LPP-1) has been studied by electron microscopy and physicochemical methods. The virion has a short (100 to 200 A long, 150 A in diameter) forked tail, with an outer sheath, an inner core, and a capital attached to one of the vertices of a polyhedral h...

journal_title：Journal of virology

authors： Luftig R,Haselkorn R

更新日期：1967-04-01 00:00:00

abstract：:The large-particle fraction from the cytoplasm of chick embryo fibroblasts infected with Semliki Forest virus was found to catalyze the incorporation of the 5'-triphosphates of guanosine, adenine, cytidine, and uridine into an acid-insoluble alkali-labile product. The conditions affecting the preparation and assay of ...

journal_title：Journal of virology

authors： Martin EM,Sonnabend JA

更新日期：1967-02-01 00:00:00